A06B-6102-H122#H520 1、高能量氧化工藝
Lee等人利用氧等離子體對PDMS晶片表面進行處理,使其表面形成帶負電的基團,然后對PDMS表面進行靜電改性,從而有效地抑制PDMS表面蛋白的非特異性吸附。Pruden等人利用氨等離子體對PDMS晶片表面進行處理,通過控制處理時間、溫度及等離子體強度,使PDMS晶片表面、氨基及亞胺基功能化。
2、動態(tài)修整
Ocvirk等人通過改變PDMS芯片微通道上對四基氧化氨(TBAC1)和十烷基硫酸鈉(SDS)進行修飾,以改變其表 面電勢,從而有效控制電滲。對非離子型表面活性劑Tween 20對PDMS芯片微通道進行動態(tài)修飾。因為Tween 20分子內(nèi)部的疏水鏈可以通過疏水作用來固定在PDMS芯片的微通道表面,而其分子中的P氧乙烯鏈部分可形成親水性的網(wǎng)狀結(jié)構(gòu)排布,該方法能較好地抑制電滲流,同時還能有效地抑制芯片微通道上氨基酸的非特異性吸附,實現(xiàn)四個氨基酸的高效、快速分離。
3,本體修整
Luo等人將碳煉酸(UDA)作為添加劑與液體PDMS混合固化,從而制備出本體修飾的PDMS芯片,提高分析物的分離效率,并改善峰展。在PDMS芯片制造過程中,肖艷等將聚乳酸聚乙醇作為添加劑加入到PDMS芯片預(yù)聚體中,實現(xiàn)了摻雜改性。研究發(fā)現(xiàn),本體修飾的PDMS芯片表面EOF和接觸角均有下降,能有效地抑制PDMS蛋白的非特異性吸附,并成功地用于兩種氨基酸的有效分離。
4溶膠單凝膠工藝
Bertson和ROrTlari使用金屬溶膠凝膠法修飾PDMS芯片表面 ,其過程如下:金屬醇鹽(異丙酶),異丙醇鉻,三異丁釩氧合成氨)是通過水蒸v擴散和修飾PDMS晶片通道表面,經(jīng)水解形成穩(wěn)定的金屬氧化物。井且在整個PDMS芯片中均勻分布,不產(chǎn)生任何化學反應(yīng),從而大大提高了PDMS芯片微通道表面的親水性。
5層層疊組裝修飾
Chen等人利用溶菌酶牛血清白蛋白膜對PDMS芯片進行多次組裝修飾 ,使EOF的穩(wěn)定性得到提高,實現(xiàn)了環(huán)境污染苯二胺、氨酚類、神經(jīng)遞質(zhì)多巴胺和腎上腺素的有效分離,Wang在PDMS芯片微通道中對聚烯丙基二甲基氧和聚4-苯乙烯酸鈉鹽進行了有效的分離,并對其進行了有效的分離。
6.化學氣相淀積法
Moorcroft研究,PDMS晶片經(jīng)過臭氧或紫外光處理,再通過氣相沉積技術(shù)在處理后的晶片表面沉積SiO2,以實現(xiàn)微量DNA序列的檢測。另外,Larger等人將聚五氟苯酚和對位二甲基苯相沉積在PDMS芯片表面,實現(xiàn)了對細胞的捕捉,并有效地抑制了羅丹明B在晶片表面的非特異性吸附。
7.表面共價嫁接
Hu等人在PDMS芯片微通道上嫁接了丙烯酸、酞胺、二甲基酞、聚(乙烯基乙二醇)丙烯酸酯和聚(乙烯基乙醇甲 氧基丙烯酸脂等,使晶片通道表面變得親水性,并保持30小時左右。Tseng等通過使用H20 :的氧化反應(yīng)和硅烷化處理PDMS芯片表面,可以成功地修飾具有氨基的PVA。聚乙烯醇修飾的PDMS芯片表面具有長時間的穩(wěn)定性,可以應(yīng)用于生物分子的固定,抑制蛋白的非特異性吸附。
A06B-6102-H122#H520
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A06B-6102-H122#H520